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Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

《农业科学与工程前沿（英文）》 2015年 第2卷 第3期   页码 260-265 doi: 10.15302/J-FASE-2015070

摘要： Y73 is a progeny of asymmetric somatic hybridization between cv. Dalixiang and the wild rice species . Inoculation with a range of strains of pv. showed that Y73 had inherited a high level of resistance to rice bacterial blight (BB) from its wild parent. An F population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar IR24. After testing 615 SSR and STS markers covering the 12 rice chromosomes, 186 markers were selected that showed polymorphism between Y73 and IR24. Molecular markers linked to the BB resistance genes in Y73 were scanned using the F population and the polymorphic markers. The SSR marker RM128 on chromosome 1, the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.

关键词： Oryza meyeriana     bacterial blight resistance gene     genetic population     linkage molecular marker    

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Bacteriophages in water pollution control: Advantages and limitations

《环境科学与工程前沿（英文）》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1378-y

摘要：

•Phages can be better indicators of enteric viruses than fecal indicator bacteria.

关键词： Phage     Water pollution monitoring     Harmful bacteria biocontrol     Horizontal gene transfer     Bacterial resistance    

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Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water: A review

Kun Wan, Wenfang Lin, Shuai Zhu, Shenghua Zhang, Xin Yu

《环境科学与工程前沿（英文）》 2020年 第14卷 第1期 doi: 10.1007/s11783-019-1189-1

摘要： Published data was used to analyze the fate of ARGs in water treatment. Biomass removal leads to the reduction in absolute abundance of ARGs. Mechanism that filter biofilm maintain ARB/ARGs was summarized. Potential BAR risks caused by biofiltration and chlorination were proposed. The bacterial antibiotic resistome (BAR) is one of the most serious contemporary medical challenges. The BAR problem in drinking water is receiving growing attention. In this study, we focused on the distribution, changes, and health risks of the BAR throughout the drinking water treatment system. We extracted the antibiotic resistance gene (ARG) data from recent publications and analyzed ARG profiles based on diversity, absolute abundance, and relative abundance. The absolute abundance of ARG was found to decrease with water treatment processes and was positively correlated with the abundance of 16S rRNA (r2 = 0.963, p<0.001), indicating that the reduction of ARG concentration was accompanied by decreasing biomass. Among treatment processes, biofiltration and chlorination were discovered to play important roles in shaping the bacterial antibiotic resistome. Chlorination exhibited positive effects in controlling the diversity of ARG, while biofiltration, especially granular activated carbon filtration, increased the diversity of ARG. Both biofiltration and chlorination altered the structure of the resistome by affecting relative ARG abundance. In addition, we analyzed the mechanism behind the impact of biofiltration and chlorination on the bacterial antibiotic resistome. By intercepting influent ARG-carrying bacteria, biofilters can enrich various ARGs and maintain ARGs in biofilm. Chlorination further selects bacteria co-resistant to chlorine and antibiotics. Finally, we proposed the BAR health risks caused by biofiltration and chlorination in water treatment. To reduce potential BAR risk in drinking water, membrane filtration technology and water boiling are recommended at the point of use.

关键词： Drinking water treatment     Antibiotic resistance gene     Biofiltration     Chlorination    

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Breeding for the resistance to

Hongxiang MA, Xu ZHANG, Jinbao YAO, Shunhe CHENG

《农业科学与工程前沿（英文）》 2019年 第6卷 第3期   页码 251-264 doi: 10.15302/J-FASE-2019262

摘要：

With the changes of climate and cultivation systems, the head blight (FHB) epidemic area in China has extended since 2000 from the reaches of the Yangtze River to the north and west winter wheat region. Breeding for FHB resistance in wheat is an effective way to control the disease. Chinese wheat breeders commenced research on FHB in the 1950s. Sumai 3, Ning 7840, Yangmai 158, Ningmai 9 and other cultivars with improved FHB resistance were developed through standard breeding methods and widely applied in production or breeding programs. In addition to intervarietal crosses, alien germplasm was used to improve FHB resistance of wheat. Addition, substitution and translocation lines with alien chromosomes or chromosome fragments were created to enhance FHB resistance. Somaclonal variation was also used to develop a FHB resistant cv. Shengxuan 3 and other cultivars with moderate resistance to FHB were released by such methods. QTL (quantitative trait loci) for FHB resistance were characterized in cultivars originating from China. The major QTL, , was identified on chromosome 3BS in Sumai 3, Ning 894037, Wangshuibai and other Chinese resistant sources. Diagnostic molecular markers for 1 have been applied in wheat breeding and breeding lines with improved FHB resistance and desirable agronomic traits have been obtained. However, breeding for FHB resistance is a long-term task, new technologies are likely to increase the efficiency of this process and better FHB resistance of new cultivars is expected to be achieved within the next decade.

关键词： breeding     Fusarium head blight     wheat    

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Effect of salinity on community structure and naphthalene dioxygenase gene diversity of a halophilicbacterial consortium

Tingting Fang, Ruisong Pan, Jing Jiang, Fen He, Hui Wang

《环境科学与工程前沿（英文）》 2016年 第10卷 第6期 doi: 10.1007/s11783-016-0888-0

摘要： Various salinities affected the PAH-biodegrading community structure. Various salinities affected the growth of strains with different salt tolerance. The two genes belonged to a new divergent cluster of the known -like genes. The two main genes had correlations with the phenanthrene degradation. The aim of this study is to analyze the effect of salinity on polycyclic aromatic hydrocarbons (PAHs) biodegradation, community structure and naphthalene dioxygenase gene ( ) diversity of a halophilic bacterial consortium with the denaturing gradient gel electrophoresis (DGGE) approach. The consortium was developed from oil-contaminated saline soil after enrichment for six times, using phenanthrene as the substrate. The prominent species in the bacterial consortium at all salinities were identified as halophilic bacteria , , , , and uncultured bacteria. The predominant microbes gradually changed associating with the saline concentration fluctuations ranging from 0.1% to 25% (w/v). Two alpha subunits were dominant at salinities ranging from 0.1% to 20%, while not been clearly detected at 25% salinity. Consistently, the biodegradation occurred at salinities ranging from 0.1% to 20%, while no at 25% salinity, suggesting the two genes played an important role in the degradation. The phylogenetic analysis revealed that both of the two alpha subunits were related to the classic -like gene from AN10 and PaK1, while one with identity of about 82% and the other one with identity of 90% at amino acid sequence level. We concluded that salinity greatly affected halophilic bacterial community structure and also the functional genes which were more related to biodegradation.

关键词： Phenanthrene     Halophilic bacteria     Gene diversity     Naphthalene dioxygenase genes    

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Antibiotic resistome mostly relates to bacterial taxonomy along a suburban transmission chain

《环境科学与工程前沿（英文）》 2022年 第16卷 第3期 doi: 10.1007/s11783-021-1466-7

摘要：

• The α-diversities of resistome were lower in manure and compost than in soils.

关键词： Antibiotic resistance genes     Resistome     Bacterial taxonomy     Transmission chain    

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我国细菌耐药问题的现状和防控策略

夏照帆,吕开阳,汤陈琪,李骏强

《中国工程科学》 2017年 第19卷 第2期   页码 106-111 doi: 10.15302/J-SSCAE-2017.02.018

摘要：

细菌耐药危机日趋严峻，已经成为全球范围内的重大公共安全问题，严重危害人类、动物的健康和生态环境。本文总结了我国各领域细菌耐药问题的现状，分析了加剧细菌耐药性的直接原因，揭示了细菌耐药问题对国民健康、食品安全、生态环境、经济发展等可能造成的危害，整理了我国目前开展的防控行动以及取得的主要成果，最后针对我国细菌耐药防控在耐药监测、抗生素监管、危害风险评估、人才队伍建设等方面的不足，提出了多领域合作、多部门联防联控的细菌耐药防控策略。

关键词： 细菌耐药     抗生素     可持续发展     防控策略    

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Assessment of antibiotic resistance genes in dialysis water treatment processes

Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1136-1

摘要：

• Quantitative global ARGs profile in dialysis water was investigated.

• Totally 35 ARGs were found in the dialysis treatment train.

• 29 ARGs (highest) were found in carbon filtration effluent.

• erm and mtrD-02 occurred in the final effluent.

• The effluent was associated with health risks even after RO treatment.

关键词： Dialysis water     Treatment process     Antibiotic resistance gene     High-throughput quantitative PCR     Horizontal gene transfer    

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The antibiotic resistome: gene flow in environments, animals and human beings

null

《医学前沿（英文）》 2017年 第11卷 第2期   页码 161-168 doi: 10.1007/s11684-017-0531-x

摘要：

The antibiotic resistance is natural in bacteria and predates the human use of antibiotics. Numerous antibiotic resistance genes (ARGs) have been discovered to confer resistance to a wide range of antibiotics. The ARGs in natural environments are highly integrated and tightly regulated in specific bacterial metabolic networks. However, the antibiotic selection pressure conferred by the use of antibiotics in both human medicine and agriculture practice leads to a significant increase of antibiotic resistance and a steady accumulation of ARGs in bacteria. In this review, we summarized, with an emphasis on an ecological point of view, the important research progress regarding the collective ARGs (antibiotic resistome) in bacterial communities of natural environments, human and animals, i.e., in the one health settings. We propose that the resistance gene flow in nature is “from the natural environments” and “to the natural environments”; human and animals, as intermediate recipients and disseminators, contribute greatly to such a resistance gene “circulation.”

关键词： antibiotic resistance     resistome     microbiome     gene flow    

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Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1127-2

摘要：

Sludge digestion is critical to control the spread of ARGs from wastewater to soil.

Fate of ARGs in three pretreatment-AD processes was investigated.

UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process.

The total ARGs concentration showed significant correlation with 16S rRNA gene.

The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.

关键词： Tetracycline resistance genes     Sulfonamide resistance genes     16S rRNA gene     Sludge pretreatment     Anaerobic sludge digestion    

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Development and testing of a weather-based model to determine potential yield losses caused by potato late blight

Alexey FILIPPOV, Maria KUZNETSOVA, Alexander ROGOZHIN, Olga IAKUSHEVA, Valentina DEMIDOVA, Natalia STATSYUK

《农业科学与工程前沿（英文）》 2018年 第5卷 第4期   页码 462-468 doi: 10.15302/J-FASE-2018239

摘要：

Late blight is one of the most important potato diseases. To minimize yield losses, various protective measures are used including fungicide application. Active use of fungicides results in a contamination of the environment. Therefore, crop protection strategies optimizing the number of treatments are of great interest. Using information about late blight development in an experimental potato field recorded over 30 seasons, a simulator to forecast yield losses caused by the disease was developed based on the number of 5-d periods favorable for reinfection of plants during a vegetation season. The simulator was successfully verified using independent data on the disease development from nine unprotected potato fields in the Netherlands and Germany. The average difference between the calculated and real yield losses did not exceed 5%. Using the simulator and weather data for a period of 2007-2017, yield losses were calculated for several areas of the Bryansk, Tambov, and Orenburg Regions of Russia. The results revealed differences in disease development between these regions and may be used to develop recommendations for a frequency of fungicide applications according to the regional risk of epidemics, leading to a significant reduction in fungicide use.

关键词： potato     late blight     Phytophthora infestans     yield losses     retrospective analysis     mathematical model    

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Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence

《环境科学与工程前沿（英文）》 2023年 第17卷 第7期 doi: 10.1007/s11783-023-1682-4

摘要：

● Abundance of MAGs carrying ARG-VF pairs unchanged in rivers after WWTP upgrade.

关键词： Wastewater treatment plant upgrade     Antibiotic resistance genes (ARGs)     Virulence factors (VFs)     Gene co-occurrence     Genome-centric analysis    

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Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

《环境科学与工程前沿（英文）》 2016年 第10卷 第3期   页码 522-530 doi: 10.1007/s11783-015-0779-9

摘要： Antibiotic resistance is a serious public health risk that may spread via potable and reclaimed water. Effective disinfection is important for inactivation of antibiotic-resistant bacteria and disruption of antibiotic resistance genes. Ampicillin is a widely prescribed antibiotic but its effectiveness is increasingly undermined by resistance. In this study, changes in ampicillin resistance for ( ) CGMCC 1.1595 were analyzed after exposure to different doses of ultraviolet (UV) or chlorine, and damage incurred by the plasmid encoding ampicillin resistance gene was assessed. We reported a greater stability in ampicillin-resistant CGMCC 1.1595 after UV irradiation or chlorination when compared with previously published data for other strains. UV irradiation and chlorination led to a shift in the mortality frequency distributions of ampicillin-resistant when subsequently exposed to ampicillin. The ampicillin hemi-inhibitory concentration (IC ) without disinfection was 3800 mg·L , and an increment was observed after UV irradiation or chlorination. The IC of ampicillin-resistant was 1.5-fold higher at a UV dose of 40 mJ·cm , and was 1.4-fold higher when exposed to 2.0 mg·L chlorine. These results indicate that UV irradiation and chlorination can potentially increase the risk of selection for strains with high ampicillin resistance. There was no evident damage to after 1–10 mg Cl ·L chlorination, while a UV dose of 80 mJ·cm yielded a damage ratio for of approximately 1.2-log. Therefore, high UV doses are required for effective disruption of antibiotic resistance genes in bacteria.

关键词： antibiotic resistance     Escherichia coli     ampicillin resistance gene     ultraviolet irradiation     chlorination    

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Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan

《医学前沿（英文）》 2007年 第1卷 第3期   页码 269-273 doi: 10.1007/s11684-007-0051-1

摘要： The aim of this article was to investigate the influence and the related mechanism of the gene on glucose uptake and insulin resistance in 3T3-L1 cells. Radioimmunoassay was used to determine glucose uptake in 3T3-L1 cells with different gene expression levels, whether cells were stimulated by insulin or not. RT-PCR and real-time RT-PCR analysis were used to determine the mRNA levels of several glucose transport proteins in 3T3-L1 cells with different gene expression levels, including insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase (PI-3K), AKT-2, glucose transporter-4 (GLUT-4), p38 mitogen-activated protein kinase (p38MAPK) and glycogen synthase kinase-3b (GSK-3). The glucose uptake decreased with the increase in gene expression in 3T3-L1 cells, which was independent of whether the cells were stimulated by insulin or not. The mRNA expression of two signal proteins PI-3K and AKT-2 decreased and the other two signal proteins, GSK-3 and p38MAPK, increased with overexpression in 3T3-L1 cells. Resistin could induce insulin resistance in adipocytes, which might be related to the changes of some proteins in PI-3K and Ras pathways.

关键词： 3T3-L1     influence     resistance     receptor substrate-1     transport    

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初生多肽相关复合物调控小麦禾谷镰刀菌生长发育和致病性 Article

王旭丽, 谢鑫, 刘敬, 王国梁, 邱德文

《工程（英文）》 2020年 第6卷 第5期   页码 546-552 doi: 10.1016/j.eng.2019.07.025

摘要：

对植物病原菌而言，维持蛋白质内稳态对其生存和繁殖至关重要。然而，当前对参与维持蛋白质内稳态的因子在真菌致病过程中的作用仍缺乏科学的认识。在本研究中，我们鉴定了十大病原真菌之一—禾谷镰刀菌（Fusarium graminearum）的初生多肽相关复合物（FgNAC）的功能。研究发现，FgNAC中的α亚基（FgNACα）与其在酵母和其他物种中的同源基因在结构和功能方面表现出高度相似性。禾谷镰刀菌NACα缺失突变体虽可存活但其菌丝生长、分生孢子产生和致病性受到严重削弱。此外，通过酵母双杂交实验发现FgNACα可以和FgNACβ亚基发生互作。亚细胞定位结果表明FgNACα和FgNACβ主要发生在细胞质中。未来的研究应致力于解析初生多肽相关复合物调节蛋白质代谢从而调控真菌发育和致病性的作用机制。

关键词： 小麦赤霉病     初生多肽相关复合物     基因敲除     致病性     亚细胞定位    

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标题 作者 时间 类型 操作

Identification of molecular markers linked to rice bacterial blight resistance genes from

Jing WANG,Chen CHENG,Yanru ZHOU,Yong YANG,Qiong MEI,Junmin LI,Ye CHENG,Chengqi YAN,Jianping CHEN

期刊论文

Bacteriophages in water pollution control: Advantages and limitations

期刊论文

Biofiltration and disinfection codetermine the bacterial antibiotic resistome in drinking water: A review

Kun Wan, Wenfang Lin, Shuai Zhu, Shenghua Zhang, Xin Yu

期刊论文

Breeding for the resistance to

Hongxiang MA, Xu ZHANG, Jinbao YAO, Shunhe CHENG

期刊论文

Effect of salinity on community structure and naphthalene dioxygenase gene diversity of a halophilicbacterial consortium

Tingting Fang, Ruisong Pan, Jing Jiang, Fen He, Hui Wang

期刊论文

Antibiotic resistome mostly relates to bacterial taxonomy along a suburban transmission chain

期刊论文

我国细菌耐药问题的现状和防控策略

夏照帆,吕开阳,汤陈琪,李骏强

期刊论文

Assessment of antibiotic resistance genes in dialysis water treatment processes

Xuan Zhu, Chengsong Ye, Yuxin Wang, Lihua Chen, Lin Feng

期刊论文

The antibiotic resistome: gene flow in environments, animals and human beings

null

期刊论文

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

期刊论文

Development and testing of a weather-based model to determine potential yield losses caused by potato late blight

Alexey FILIPPOV, Maria KUZNETSOVA, Alexander ROGOZHIN, Olga IAKUSHEVA, Valentina DEMIDOVA, Natalia STATSYUK

期刊论文

Mitigating microbiological risks of potential pathogens carrying antibiotic resistance genes and virulence

期刊论文

Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

期刊论文

Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells

LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan

期刊论文

初生多肽相关复合物调控小麦禾谷镰刀菌生长发育和致病性

王旭丽, 谢鑫, 刘敬, 王国梁, 邱德文

期刊论文